Journal: Experimental Biology and Medicine

Article Title: Curcumin-enhanced elvitegravir therapy mitigates neuroinflammation and cognitive deficits in EcoHIV mice

doi: 10.3389/ebm.2025.10758

Figure Lengend Snippet: Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers NeuN, TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.

Article Snippet: Primary antibody incubation was carried out overnight at 4 °C using the following antibodies: NeuN rabbit polyclonal antibody (1:1000, Proteintech, Cat# 26975-1-AP), synaptophysin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 67864-1-Ig), GFAP rabbit polyclonal antibody (1:1000), L1CAM rabbit polyclonal antibody (Proteintech, Cat# 20659-1-AP), and β-actin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 66009-1-Ig) as an internal loading control.

Techniques: Infection, Western Blot, Expressing

Journal: Brain Sciences

Article Title: Topographical Organization of Prefrontal Cortex and Adjacent Areas Projections to the Dorsomedial Caudate–Putamen in Rats: A Retrograde Tracing Study

doi: 10.3390/brainsci15040398

Figure Lengend Snippet: Representative images of cells positively labeled with NeuN and Fluoro-Gold in prefrontal cortex. ( A ) NeuN-positive cells at 20× magnification. ( B ) Magnified view of subsection outlined by a white rectangle in panel ( A ), showing NeuN-positive cells in detail. ( C ) Fluoro-Gold-positive cells at 20× magnification. ( D ) Magnified view of section outlined by a white rectangle in panel ( C ), displaying Fluoro-Gold-positive cells in detail.

Article Snippet: Briefly, sections were washed in 0.02 M PBS (3 × 10 min), blocked with 10% normal goat serum in 0.02 M PBS containing 0.1% Triton X-100 for 1 h at room temperature, and incubated with a 1:5000 dilution of polyclonal rabbit anti-NeuN antibody (ABN78; EMD Millipore; Temecula, CA, USA) for 24 h at 4 °C.

Techniques: Labeling